Optical Pooled Multi-omic Single Cell Screening

Dr. Jellert T. Gaublomme

Assistant Professor, Biological Sciences, Columbia University, NY

November 28, 2022 | 3:30 PM

Ammon-Pinizzotto Biopharmaceutical Innovation (BPI) Building
Conference Room 140

Pooled CRISPR screens enable perturbation studies of pathways at scale, and with
single-cell resolution. Until recently the phenotypic readout associated with
pooled screens was typically limited to fitness, or the expression of a tagged
marker protein. Single-cell RNA-sequencing vastly expanded our readout to the
whole transcriptome, but often relies on lysis and is thus oblivious to cell
morphology and subcellular protein localization patterns as a function of the
incurred CRISPR perturbation. Here, we show our development of a multi-omic
optical pooled screening approach that combines in situ RNA, protein and
barcode readout at the single-cell level.

BIOGRAPHY

Jellert Gaublomme is an Assistant Professor in the department of Biological
Sciences at Columbia University. His lab develops and applies multi-omic
technologies to study biomolecular and cellular interactions. The application
areas include immunology and cancer. He received his PhD in 2014 from
Harvard University with Professor Hongkun Park, and subsequently did a
postdoctoral fellowship at the Broad Institute with Professor Aviv Regev.

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